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The assay consists in introducing a 2 ml water sample into the ready to use cuvette and after a 15 minutes preincubation to perform read-outs using the provided fluorimeter at time zero, 30 and 60 minutes. The higher the concentration of toxin the soonner the alarm of the fluorimeter will ring. For very high toxin concentrations, the alarm will ring at time zero. The assay can detect concentrations as low as 5 ng/mL of 150kDa toxin (ref: Pasteur Institute Toxin A and Pr Jonhson Toxin Type B).
No cumbersome handlings and no specific skills are required to perform the assay.
If a positive measurement is identified, a warning is displayed on the fluorimeter screen together with a buzzer.
Due to the lethality of botulinum toxins, PHARMALEADS thought it relevant to provide an EDTA confirmation test. EDTA is a powerful inhibitor of zinc metallopeptidases, family to which belong all types of botulinum toxins.
ln the case of a positive result and to confirm that the contaminated sample does contain botulinum toxin, the same test can be performed adding EDTA. A negative result in the presence of EDTA will confirm the presence of botulinum toxin in the incriminated sample.
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